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Hoefig, Ines; Ehrhardt, Harald; Jeremias, Irmela: Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice. In: Cell Communication and Signaling 2012, 10:8




Background: Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice. Results: We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple genes reduced constitutive or induced protein expression accompanied by marked signaling alterations. Conclusion: Our novel technique allows using patient-derived tumor cells instead of cell lines for signaling studies in leukemia.