Förster energy transfer between neighbouring chromophores in C-phycocyanin trimers

Gillbro, T.; Sharkov, A. V.; Kryukov, I. V.; Khoroshilov, E. V.; Kryukov, P. G.; Fischer, R. und Scheer, Hugo (1993): Förster energy transfer between neighbouring chromophores in C-phycocyanin trimers. In: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Bd. 1140, Nr. 3: S. 321-326

Abstract

The excitation-energy transfer in C-phycocyanin (C-PC) trimers and monomers isolated from phycobilisomes of Mastigocladus laminosus has been studied by polarization femtosecond laser spectroscopy. Excitation with 70-fs pulses at 615 nm gave rise to a 500-fs energy-transfer process that was observed only in trimeric preparations. The rate of the process is in agreement with earlier calculated Förster energy transfer rates between neighbouring α-84 and β-84 chromophores of different monomeric subunits. This process is most clearly seen in the anisotropy decay kinetics. As a result of femtosecond excitation-energy transfer, the anisotropy relaxes from 0.4 to 0.23. The final anisotropy value is in fair agreement with the results of calculations based on the crystal structure and spectroscopic data of C-PC trimers. Our results support the conclusion that Förster energy transfer can occur between excitonically coupled chromophores.

• Dokumententyp: Zeitschriftenartikel
• Keywords: Phycocyanin; Excitation energy transfer; Femtosecond spectroscopy; Anisotropy; Photosynthesis
• Fakultät: Biologie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie