Limantara, Leenawaty; Koyama, Yasushi; Katheder, Ingrid; Scheer, Hugo
Transient Raman spectroscopy of 15N-substituted bacteriochlorophyll a. An empirical assignment of T1 Raman lines.
In: Chemical Physics Letters, Vol. 227, Nr. 6: S. 617-622
15N-substituted bacteriochlorophyll a (BChl a) was extracted from the cells of Rhodobacter sphaeroides 2.4.1 grown in a medium containing 15N-ammonium sulfate and yeast concentrate. The T1 Raman spectra of 14N-and 15N-BChl a were obtained as the difference spectra of high-power minus low-power of one-color, pump-and-probe measurements using 420 nm, 5 ns pulses. A set of empirical assignments of the T1 Raman lines was made, based on shifts upon 14N→15N substitution. The S0 Raman spectra of the two BChls were also obtained by using the 457.9 nm cw beam, and a set of assignments of the S0 Raman lines was given for comparison.