Friedrich, J.; Scheer, Hugo; Zickendraht-Wendelstadt, Barbara; Haarer, D.
High-Resolution Optical Studies on C-Phycocyanin via Photochemical Hole Burning.
In: Journal of the American Chemical Society, Vol. 103: pp. 1030-1035
We have shown that both the native C-phycocyanin and its corresponding free biline chromophore undergo reversible,
low-temperature photochemistry. We attribute this photochemistry to reversible proton-transfer processes and utilize the observed photoreaction for photochemical hole burning (PHB). Using narrow-band PHB experiments, we have been able to perform high-resolution optical studies and show that the protein-chromophore assembly forms a very rigid structure. The results lead to the conclusion that the light-induced proton transfer occurs most probably in the triplet state.