Lopez de Haro, Maria S.; Salgado, Luis M.; David, Charles N.; Bosch, Thomas C. G.
Hydra tropomyosin TROP1 is expressed in head-specific epithelial cells and is a major component of the cytoskeletal structure that anchors nematocytes.
In: Journal of Cell Science, Vol. 107: pp. 1403-1411
A cDNA clone encoding a 253 amino acid tropomyosin was
Hydra in a differential screen for headspecific
genes. The Hydra tropomyosin gene, designated
trop1, is a single copy gene, lacks introns and is strongly
expressed in tentacle-specific epithelial cells. Analysis of
protein synthesis in head and gastric tissue indicated a high
rate of tropomyosin synthesis in head tissue. Immunolocalization
of tropomyosin in tentacle tissue revealed a
cushion-like tropomyosin-containing structure within
battery cells at the base of nematocytes. The structure
appears to form part of the cytoskeletal anchor for nematocytes.
Tropomyosin cushions were also observed in
epithelial cells along the body column, which contain
mounted stenotele nematocytes.