Fujisawa, Toshitaka; David, Charles N.
Commitment during stenotele differentiation in Hydra is localized near the S/G2 boundary in the terminal cell cycle.
In: Developmental Biology, Vol. 93, No. 1: pp. 226-230
The timing of commitment during stenotele differentiation in Hydra was determined. Regeneration of isolated distal regions of the body column induces stenotele differentiation. The kinetics of appearance of committed stenotele precursors was determined in such regenerating pieces. Using [3H]thymidine labeling and hydroxyurea sensitivity, the G1/S and the S/G2 boundaries of the precursor population was also determined. Comparison of these results indicates that stenotele commitment is localized near the S/G2 boundary in the terminal cell cycle of nests of precursor cells.