Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae

Schreiber, Konrad; Csaba, Gergely; Haslbeck, Martin und Zimmer, Ralf (2015): Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae.
In: PLOS ONE 10(10), e0140487

Abstract

mRNA splicing is required in about 4% of protein coding genes in Saccharomyces cerevisiae. The gene structure of those genes is simple, generally comprising two exons and one intron. In order to characterize the impact of alternative splicing on the S. cerevisiae transcriptome, we perform a systematic analysis of mRNA sequencing data. We find evidence of a pervasive use of alternative splice sites and detect several novel introns both within and outside protein coding regions. We also find a predominance of alternative splicing on the 3' side of introns, a finding which is consistent with existing knowledge on conservation of exon-intron boundaries in S. cerevisiae. Some of the alternatively spliced transcripts allow for a translation into different protein products.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Mathematik, Informatik und Statistik > Informatik
• Themengebiete: 000 Informatik, Informationswissenschaft, allgemeine Werke > 004 Informatik
• ISSN: 1932-6203
• Sprache: Englisch