Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry

Weiss, Frederik; Schnabel, Anke; Planatscher, Hannes; Berg, Bart H. J. van den; Serschnitzki, Bettina; Nuessler, Andreas K.; Thasler, Wolfgang E.; Weiss, Thomas S.; Reuss, Matthias; Stoll, Dieter; Templin, Markus F.; Joos, Thomas O.; Marcus, Katrin and Poetz, Oliver (2015): Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry. In: Scientific Reports, Vol. 5, 8759

Abstract

Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of peptides, thus facilitating multiplexed quantitative protein assays. We tested this strategy in a pharmacokinetic experiment by targeting a group of homologous drug transforming proteins in human hepatocytes. Our results indicate the generic applicability of this method to any biological system.

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 2045-2322
• Language: English