Ammonia sensing for enzymatic urea detection using organic field effect transistors and a semipermeable membrane

Werkmeister, Franz; Koide, T. und Nickel, Bert (2015): Ammonia sensing for enzymatic urea detection using organic field effect transistors and a semipermeable membrane. In: Journal of Materials Chemistry B, Bd. 4, Nr. 1: S. 162-168

Abstract

Organic Field Effect Transistors (OFETs) are used to measure ammonia in solution via ammonia diffusion into the OFET channel. An increase in ammonia concentrations results in a decrease in transistor currents. The regeneration of the OFET current after ammonia uptake is slow, which allows us to read out the maximum ammonia dose which was applied. A 100 nm parylene-C layer serves as a semipermeable top gate dielectric. The parylene layer is functionalized with the covalently attached enzyme urease. The enzyme catalyses the hydrolysis of urea to ammonia and carbon dioxide, i.e. urea can be detected via its hydrolysis product ammonia. The sensitivity covers a range of physiological concentrations of urea, which are several mM.

• Dokumententyp: Zeitschriftenartikel
• Publikationsform: Publisher's Version
• Fakultät: Physik
• Themengebiete: 500 Naturwissenschaften und Mathematik > 530 Physik
• ISSN: 0959-9428
• Allianz-/Nationallizenz: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
• Sprache: Englisch