Identification of a Candidate CD5 Homologue in the Amphibian Xenopus laevis

Jürgens, Jutta B.; Gartland, Lanier A.; Pasquier, Louis D.; Horton, John D.; Göbel, Thomas W. F. und Cooper, Max D. (1995): Identification of a Candidate CD5 Homologue in the Amphibian Xenopus laevis. In: Journal of Immunology, Bd. 155, Nr. 9: S. 4218-4223

Abstract

We identified a novel T cell Ag in the South African clawed toad (Xenopus laevis) by a mAb designated 2B1. This Ag is present in relatively high levels on most thymocytes, approximately 65% of splenocytes, 55% of PBL, and 65% of intestinal lymphocytes, but is rarely seen on IgM+ B cells in any of these tissues. Lymphocytes bearing the 2B1 Ag proliferate in response to stimulation with Con A or PHA, whereas the 2B1- lymphocytes are reactive to LPS. Biochemical analysis indicates that this Ag is a differentially phosphorylated glycoprotein of 71 to 82 kDa. The protein core of 64 kDa bears both N- and O-linked carbohydrate side chains. The amino-terminal protein sequence of the 2B1 Ag shares significant homology with both the macrophage scavenger receptor type 1 motif and the mammalian CD5/CD6 family. The biochemical characteristics and cellular distribution of the 2B1 Ag suggest that it represents the CD5 homologue in X. laevis. While T cells constitutively express this highly conserved molecule, Xenopus B cells acquire the CD5 homologue only when they are stimulated in the presence of T cells

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Tiermedizin
• Themengebiete: 500 Naturwissenschaften und Mathematik > 590 Tiere (Zoologie)
• ISSN: 0022-1767