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Soll, Jürgen; Kemmerling, Monika; Schultz, Gernot (1980): Tocopherol and plastoquinone synthesis in spinach chloroplasts subfractions. In: Archives of Biochemistry and Biophysics, Vol. 204, Nr. 2: S. 544-550
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Abstract

Subfractions isolated from intact purified spinach chloroplasts are able to prenylate the aromatic moiety of -tocopherol and plastoquinone-9 precursors. The biosynthesis of -tocopherol and plastoquinone-9 is a compartmentalized process. The chloroplast envelope membranes are the only site of the enzymatic prenylation in -tocopherol synthesis whereas the thylakoid membrane is also involved in the prenylation and methylation sequence of plastoquinone-9 biosynthesis. A very active kinase which forms phytyl-PP is localized in the stroma. Phytol but not geranylgeraniol is the polyprenol precursor of the side chain of -tocopherol in spinach chloroplasts.