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Hinterseher, C.; Reese, Sven ORCID: 0000-0002-4605-9791; Kaspers, Bernd (2007): Lektinhistochemische Untersuchungen der Hühnerlunge zur Verbesserung der praktischen pulmonalen Vakzinierung in der Aviärmedizin. In: Tierärztliche Praxis / K, Kleintiere, Vol. 35, No. 4: pp. 292-296
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Abstract

Objective: Carbohydrates on the surface of the lung-associated epithelium may serve as receptors for a variety of microorganisms.Material and methods: For this reason lectin histochemistry was applied on different lung tissue segments of chickens (Gallus gallus domesticus) at the age of three days, one, two ,three, 10 and 11 weeks. 24 biotinylated lectins with five different carbohydrate-specifities were used for histochemistry on paraffin-embedded tissue of lung epithelial cells. Results: In the primary bronchi cells of the respiratory epithelium were stained with the GaINAc-specifity (GIcNAc) lectins SBA, VVA und ECL. Epithelial cells of the secondary bronchi and the atria could be detected by the GIcNAc-lectins WGA and s-WGA. Conclusion: These studies provide the basis for further investigations into structure-function relationships at the respiratory epithelium and studies aimed at understanding of host-pathogen interaction at this crucial inner surface. Clinical relevance: The development of new and highly specific markers will allow advanced studies on the ontogeny of the follicle-associated epithelium and the associated lymphoid structures and development of more effective oral or oronasal vaccination products.