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Rüffer, Martina and Zenk, Meinhart H. (1987): Enzymatic formation of protopines by a microsomal cytochrome P-450 system of Corydalis vaginans. In: Tetrahedron Letters, Vol. 28, No. 44: pp. 5307-5310 [PDF, 217kB]


A microsomal cytochrome P-450-NADPH dependent enzyme which hydroxylates stereo- and regiospecifically carbon atom 14 of (S)- -N- methyltetrahydroprotoberberines has been discovered in a number of plant cell cultures originating from species containing protopine alkaloids; the monooxygenase was solubilized, partially purified (100-fold) and characterized.

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