Biel, Martin; Zong, Xiangang; Distler, Madeleine; Bosse, Eva; Klugbauer, Norbert; Murakami, Manabu; Flockerzi, Veit; Hofmann, Franz
Another member of the cyclic nucleotide-gated channel family, expressed in testis, kidney, and heart.
In: Proceedings of the National Academy of Science of the U S A, Vol. 91, Nr. 9: S. 3505-3509
Cyclic nucleotide-gated cation channels are essential in visual and olfactory signal transduction. An additional member of the cGMP-gated channel family, termed CNG-3, has been cloned from bovine kidney. Its deduced amino acid sequence is 60% and 62% identical with the CNG-channel proteins from bovine rod outer segment and bovine olfactory epithelium, respectively. Northern analysis and sequences amplified by the PCR showed that the CNG-3 mRNA is present in testis, kidney, and heart. Calcium permeated the expressed channel in the presence of extracellular Mg2+ and Na+ at membrane potentials from -100 to +45 mV. It is likely that CNG-3 protein is responsible for cGMP-induced Ca2+ entry in cells other than sensory cells.