Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome

Griffin, Darren K.; Habermann, Felix A.; Masabanda, J.; O'Brien, P.; Bagga, M.; Sazanov, A.; Smith, J.; Burt, D. W.; Ferguson-Smith, M. A. und Wienberg, J. (1999): Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome. In: Cytogenet Cell Genet, Bd. 87, Nr. 3-4: S. 278-281

Abstract

Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.

• Dokumententyp: Zeitschriftenartikel
• Publikationsform: Publisher's Version
• Fakultät: Tiermedizin ; Tiermedizin > Veterinärwissenschaftliches Department ; Tiermedizin > Veterinärwissenschaftliches Department > Lehrstuhl für Anatomie, Histologie und Embryologie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 590 Tiere (Zoologie) ; 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Sprache: Englisch