Wittmann, Anke; Grimm, Marcus O. W.; Scherthan, Harry; Horsch, Marion; Beckers, Johannes; Fuchs, Helmut; Gailus-Durner, Valerie; de Angelis, Martin Hrabe; Ford, Steven J.; Burton, Neal C.; Razansky, Daniel; Trümbach, Dietrich; Aichler, Michaela; Walch, Axel Karl; Calzada-Wack, Julia; Neff, Frauke; Wurst, Wolfgang; Hartmann, Tobias; Floss, Thomas
(2016):
Sphingomyelin Synthase 1 Is Essential for Male Fertility in Mice.
In: PLOS ONE 11(10), e0164298 |
| 5MB |
Abstract
Sphingolipids and the derived gangliosides have critical functions in spermatogenesis, thus mutations in genes involved in sphingolipid biogenesis are often associated with male infertility. We have generated a transgenic mouse line carrying an insertion in the sphingomyelin synthase gene Sms1, the enzyme which generates sphingomyelin species in the Golgi apparatus. We describe the spermatogenesis defect of Sms1(-/-) mice, which is characterized by sloughing of spermatocytes and spermatids, causing progressive infertility of male homozygotes. Lipid profiling revealed a reduction in several long chain unsaturated phosphatidylcholins, lysophosphatidylcholins and sphingolipids in the testes of mutants. Multi-Spectral Optoacoustic Tomography indicated blood-testis barrier dysfunction. A supplementary diet of the essential omega-3 docosahexaenoic acid and eicosapentaenoic acid diminished germ cell sloughing from the seminiferous epithelium and restored spermatogenesis and fertility in 50% of previously infertile mutants. Our findings indicate that SMS1 has a wider than anticipated role in testis polyunsaturated fatty acid homeostasis and for male fertility.
Item Type: | Journal article |
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Faculties: | Medicine |
Subjects: | 600 Technology > 610 Medicine and health |
URN: | urn:nbn:de:bvb:19-epub-37478-5 |
ISSN: | 1932-6203 |
Language: | English |
ID Code: | 37478 |
Deposited On: | 04. May 2017 13:09 |
Last Modified: | 04. Nov 2020 14:43 |
- BASE
- Wittmann, Anke
- Grimm, Marcus O. W.
- Scherthan, Harry
- Horsch, Marion
- Beckers, Johannes
- Fuchs, Helmut
- Gailus-Durner, Valerie
- de Angelis, Martin Hrabe
- Ford, Steven J.
- Burton, Neal C.
- Razansky, Daniel
- Trümbach, Dietrich
- Aichler, Michaela
- Walch, Axel Karl
- Calzada-Wack, Julia
- Neff, Frauke
- Wurst, Wolfgang
- Hartmann, Tobias
- Floss, Thomas
- Google Scholar
- Wittmann, Anke
- Grimm, Marcus O. W.
- Scherthan, Harry
- Horsch, Marion
- Beckers, Johannes
- Fuchs, Helmut
- Gailus-Durner, Valerie
- de Angelis, Martin Hrabe
- Ford, Steven J.
- Burton, Neal C.
- Razansky, Daniel
- Trümbach, Dietrich
- Aichler, Michaela
- Walch, Axel Karl
- Calzada-Wack, Julia
- Neff, Frauke
- Wurst, Wolfgang
- Hartmann, Tobias
- Floss, Thomas