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Hamm, P.; Gray, Kevin A.; Oesterhelt, Dieter; Feick, R.; Scheer, Hugo und Zinth, Wolfgang (1993): Subpicosecond emission studies of bacterial reaction centers. In: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Nr. 1142: S. 99-105 [PDF, 646kB]

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Abstract

The spontaneous emission of reaction centers from native and mutated Rhodobacter sphaeroides and from wild type Chloroflexus aurantiacus is investigated by fluorescence up-conversion with high temporal resolution. The time constant of 0.9 ps previously observed in transient absorption experiments on wild type reaction centers of Rhodobacter sphaeroides does not appear in the emission experiment. However, all investigated reaction centers display a biexponential decay of the emission with time constants in the 2 ps to 25 ps range. The experimental results are discussed within the frame of different reaction models including a possible sample heterogeneity or a transient electron transfer to the inactive pigment branch.

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