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Rädler, Joachim O.; Radmacher, M. und Gaub, H. (1994): Velocity-Dependent Forces in Atomic Force Microscopy Imaging of Lipid Films. In: Langmuir, Nr. 10: S. 3111-3115 [PDF, 1MB]

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Abstract

We have imaged adsorbed fluid lipid bilayers by atomic force microscopy. The patches were formed by rupture of phospholipid vesicles onto magnesium fluoride. We show that the membrane patches are fluid but can be stably imaged at scan rates higher than 6 p d s . At lower scan rates the tip penetrates through the layer. The penetrating tip does not destroy the fluid patches, and the previous image can be restored after increasing the scanning velocity. The dynamic forces that possibly explain the effect are discussed.

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