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Zurita, Francisco; Galera, Teresa; Gonzalez-Paramos, Cristina; Moreno-Izquierdo, Ana; Schneiderat, Peter; Fraga, Mario F.; Fernandez, Agustin F.; Garesse, Rafael; Esther Gallardo, M. (2016): Generation of a human iPSC line from a patient with a defect of intergenomic communication. In: Stem Cell Research, Vol. 16, No. 1: pp. 120-123
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Abstract

Human iPSC line PG64SV.2 was generated from fibroblasts of a patient with a defect of intergenomic communication. This patient harbored a homozygous mutation (c.2243GNC;p.Trp748Ser) in the gene encoding the catalytic subunit of the mitochondrial DNA polymerase gamma gene (POLG). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non integrative methodology that involves the use of Sendai virus. (C) 2015 The Authors. Published by Elsevier B.V.