Mouse redox histology using genetically encoded probes

Fujikawa, Yuuta; Roma, Leticia P.; Sobotta, Mirko C.; Rose, Adam J.; Diaz, Mauricio Berriel; Locatelli, Giuseppe; Breckwoldt, Michael O.; Misgeld, Thomas; Kerschensteiner, Martin; Herzig, Stephan; Müller-Decker, Karin und Dick, Tobias P. (2016): Mouse redox histology using genetically encoded probes. In: Science Signaling, Bd. 9, Nr. 419, rs1

Abstract

Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing "redox maps" for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1945-0877
• Sprache: Englisch