Abstract
Interferon beta (IFN-beta) is a key component of cellular innate immunity in mammals, and it constitutes the first line of defense during viral infection. Studies with cultured cells previously showed that almost all nucleated cells are able to produce IFN-beta to various extents, but information about the in vivo sources of IFN-beta remains incomplete. By applying immunohistochemistry and employing conditional-reporter mice that express firefly luciferase under the control of the IFN-beta promoter in either all or only distinct cell types, we found that astrocytes are the main producers of IFN-beta after infection of the brain with diverse neurotropic viruses, including rabies virus, Theiler's murine encephalomyelitis virus, and vesicular stomatitis virus. Analysis of a panel of knockout mouse strains revealed that sensing of viral components via both RIG-I-like helicases and Toll-like receptors contributes to IFN induction in the infected brain. A genetic approach to permanently mark rabies virus-infected cells in the brain showed that a substantial number of astrocytes became labeled and, therefore, must have been infected by the virus at least transiently. Thus, our results strongly indicate that abortive viral infection of astrocytes can trigger pattern recognition receptor signaling events which result in secretion of IFN-beta that confers antiviral protection.
| Dokumententyp: | Zeitschriftenartikel |
|---|---|
| Fakultät: | Medizin |
| Themengebiete: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit |
| ISSN: | 0022-538X |
| Sprache: | Englisch |
| Dokumenten ID: | 43777 |
| Datum der Veröffentlichung auf Open Access LMU: | 27. Apr. 2018, 08:04 |
| Letzte Änderungen: | 04. Nov. 2020, 13:19 |
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