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Braunagel, Margarita; Helck, Andreas; Wagner, Anne; Schupp, Nina; Bröcker, Verena; Reiser, Maximilian; Notohamiprodjo, Mike; Meiser, Bruno; Habicht, Antje (2016): Dynamic Contrast-Enhanced Computed Tomography: A New Diagnostic Tool to Assess Renal Perfusion After Ischemia-Reperfusion Injury in Mice: Correlation of Perfusion Deficit to Histopathologic Damage. In: Investigative Radiology, Vol. 51, No. 5: pp. 316-322
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Abstract

Objective The aim of this study was to investigate the value of dynamic contrast-enhanced computed tomography (CT) in the assessment of renal perfusion parameters after ischemia-reperfusion (I/R) injury in an experimental murine model. Materials and MethodsBalb/cJ wildtype mice were subjected to 45 minutes (AKI(45)) or 60 minutes (AKI(60)) of unilateral warm I/R injury by clamping the pedicle of the right kidney. Two, 7, and 18 days after right I/R injury, renal blood flow (RBF), renal volume of distribution (RVD), and mean transit time were quantitatively assessed in the cortex of both kidneys by dynamic contrast-enhanced CT. Acute tubular injury (ATI) was assessed by histologic analysis using a semiquantitative sum score (score, 0-18) and correlated with RBF, RVD, and mean transit time. Results Histologic signs of ATI could be detected in the clamped kidneys in both groups already at day 2. Pathologic features of ATI worsened in AKI(60) until day 18 (score, 7 0), whereas mice in AKI(45) group showed amelioration over time (score, 4 +/- 2). Renal blood flow was significantly reduced in ischemic kidneys in AKI(45) (287 +/- 32 mL/100 mL per minute;P < 0.01) and AKI(60) group (249 +/- 73 mL/100 mL per minute;P < 0.01) as compared with that in healthy kidneys (402 +/- 49 mL/100 mL per minute) on day 2. It decreased further at day 7 in both groups (AKI(45): 165 +/- 44 mL/100 mL per minute, P < 0.01;AKI(60): 151 +/- 72 mL/100 mL per minute, P < 0.05) and improved at day 18 in AKI(45) (261 +/- 11 mL/100 mL per minute, P < 0.05) and to a lesser degree in AKI(60) (197 +/- 52 mL/100 mL per minute, P > 0.05). Values of RVD paralleled RBF at all time points. Renal blood flow (r = -0.79;P < 0.01) and RVD (r = -0.8;P < 0.01) significantly correlated with the histological damage score (Spearman rank correlation). Conclusions Dynamic contrast-enhanced CT is a noninvasive method to determine renal perfusion changes in acute kidney injury. It might be a valuable diagnostic tool to predict outcome or monitor treatment effects of renal I/R injury.