Cerebrospinal Fluid Real-Time Quaking-Induced Conversion Is a Robust and Reliable Test for Sporadic Creutzfeldt-Jakob Disease: An International Study

McGuire, Lynne I.; Poleggi, Anna; Poggiolini, Ilaria; Suardi, Silvia; Grznarova, Katarina; Shi, Song; Vil, Bart de; Sarros, Shannon; Satoh, Katsuya; Cheng, Keding; Cramm, Maria; Fairfoul, Graham; Schmitz, Matthias; Zerr, Inga; Cras, Patrick; Equestre, Michele; Tagliavini, Fabrizio; Atarashi, Ryuichiro; Knox, David; Collins, Steven; Haik, Stephane; Parchi, Piero; Pocchiari, Maurizio; Green, Alison (2016): Cerebrospinal Fluid Real-Time Quaking-Induced Conversion Is a Robust and Reliable Test for Sporadic Creutzfeldt-Jakob Disease: An International Study. In: Annals of Neurology, Vol. 80, No. 1: pp. 160-165

Abstract

Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease;however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT-QuIC is a suitably reliable and robust technique for clinical practice.

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 0364-5134
• Language: English