Regulatory Impact of Amniotic Membrane Transplantation on Presence of Adhesion/Growth-Regulatory Galectins-1 and-7 in Corneal Explants from Acanthamoeba Keratitis Patients: Clinical Note

Smorodinova, Natalia; Kaltner, Herbert; Jirsová, Katerina; Hrdličková-Cela, Enkela; André, Sabine; Kučera, Tomáš; Smetana, Karel und Gabius, Hans-Joachim (2016): Regulatory Impact of Amniotic Membrane Transplantation on Presence of Adhesion/Growth-Regulatory Galectins-1 and-7 in Corneal Explants from Acanthamoeba Keratitis Patients: Clinical Note. In: Current Eye Research, Bd. 41, Nr. 6: S. 740-746

Abstract

Purpose: To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence of two multifunctional endogenous lectins, i.e. galectins-1 and -7.Methods: Ten corneal explants from AK patients (five with previous AMT and five controls without this treatment) and seven specimens of disease-free control cornea were processed by indirect fluorescent immunohistochemistry.Results: Immunostaining for both galectins was obtained in the epithelium, stroma and the endothelial layer of all controls, with the strongest positivity in the epithelium. Significantly decreased intensity for galectin-1 was recorded in the epithelium of corneal explants from patients with AK and AMT. The signal for galectin-7 was significantly decreased in the epithelium of AK patients and normalized after AMT.Conclusions: AMT has a marked impact on presence of the two galectins in opposite directions, encouraging complete profiling for this family of endogenous effectors.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0271-3683
• Sprache: Englisch