Fimbrolide Natural Products Disrupt Bioluminescence of Vibrio By Targeting Autoinducer Biosynthesis and Luciferase Activity

Zhao, Weining; Lorenz, Nicola; Jung, Kirsten and Sieber, Stephan A. (2016): Fimbrolide Natural Products Disrupt Bioluminescence of Vibrio By Targeting Autoinducer Biosynthesis and Luciferase Activity. In: Angewandte Chemie-international Edition, Vol. 55, No. 3: pp. 1187-1191

Abstract

Vibrio is a model organism for the study of quorum sensing (QS) signaling and is used to identify QS-interfering drugs. Naturally occurring fimbrolides are important tool compounds known to affect QS in various organisms;however, their cellular targets have so far remained elusive. Here we identify the irreversible fimbrolide targets in the proteome of living V.harveyi and V.campbellii via quantitative mass spectrometry utilizing customized probes. Among the major hits are two protein targets with essential roles in Vibrio QS and bioluminescence. LuxS, responsible for autoinducer2 biosynthesis, and LuxE, a subunit of the luciferase complex, were both covalently modified at their active-site cysteines leading to inhibition of activity. The identification of LuxE unifies previous reports suggesting inhibition of bioluminescence downstream of the signaling cascade and thus contributes to a better mechanistic understanding of these QS tool compounds.

• Item Type: Journal article
• Faculties: Biology > Department Biology I
• Subjects: 500 Science > 570 Life sciences; biology
• ISSN: 1433-7851
• Language: English