Dexamethasone-conjugated DNA nanotubes as anti-inflammatory agents in vivo

Sellner, Sabine; Kocabey, Samet; Zhang, Tao; Nekolla, Katharina; Hutten, Saskia; Krombach, Fritz; Liedl, Tim und Rehberg, Markus (2017): Dexamethasone-conjugated DNA nanotubes as anti-inflammatory agents in vivo. In: Biomaterials, Bd. 134: S. 78-90

Abstract

The biopolymer DNA allows to create nanoscale, biocompatible structures, which can be designed in a target-specific and stimuli-responsive manner. DNA carrier systems with these characteristics hold a great potential for nanomedical applications, such as for the treatment of inflammatory diseases. Here we used a DNA-based drug carrier system for the pH-dependent delivery of the glucocorticoid dexamethasone into macrophages, a cell type with a key role in the regulation of inflammation. Dexamethasone (Dex) nanotubes were internalized within minutes by MH-S macrophages in vitro and by tissue resident macrophages in the mouse cremaster muscle in vivo and localized in their endosomes. Treatment with Dex nanotubes in vitro significantly reduced the LPS-induced TNF secretion by macrophages, as compared to equivalent amounts of free dexamethasone without affecting cell viability. Microinjection of Dex nanotubes into postischemic muscle tissue of anesthetized mice resulted in a marked reduction of ischemia-reperfusion-elicited leukocyte transmigration and diminished vascular expression of the endothelial adhesion molecules VCAM-1 and ICAM-1. Taken together, our results demonstrate that DNA nanotubes can be used as a platform for the targeted delivery of glucocorticoids and could thus foster the development of nanomedical therapeutics with reduced off-target effects.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin ; Physik
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit ; 500 Naturwissenschaften und Mathematik > 530 Physik
• ISSN: 0142-9612
• Sprache: Englisch