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Königer, Verena; Holsten, Lea; Harrison, Ute; Busch, Benjamin; Loell, Eva; Zhao, Qing; Bonsor, Daniel A.; Roth, Alexandra; Kengmo-Tchoupa, Arnaud; Smith, Stella I.; Müller, Susanna; Sundberg, Eric J.; Zimmermann, Wolfgang; Fischer, Wolfgang; Hauck, Christof R.; Haas, Rainer (2017): Helicobacter pylori exploits human CEACAMs via HopQ for adherence and translocation of CagA. In: Nature Microbiology, Vol. 2, No. 1, 16188
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Abstract

Helicobacter pylori (Hp) strains that carry the cag type IV secretion system (cag-T4SS) to inject the cytotoxin-associated antigen A (CagA) into host cells are associated with peptic ulcer disease and gastric adenocarcinoma. CagA translocation by Hp is mediated by beta 1 integrin interaction of the cag-T4SS. However, other cellular receptors or bacterial outer membrane adhesins essential for this process are unknown. Here, we identify the HopQ protein as a genuine Hp adhesin, exploiting defined members of the carcinoembryonic antigen-related cell adhesion molecule family (CEACAMs) as host cell receptors. HopQ binds the amino-terminal IgV-like domain of human CEACAM1, CEACAM3, CEACAM5 or CEACAM6 proteins, thereby enabling translocation of the major pathogenicity factor CagA into host cells. The HopQ-CEACAM interaction is characterized by a remarkably high affinity (K-D from 23 to 268 nM), which is independent of CEACAM glycosylation, identifying CEACAMs as bona fide protein receptors for Hp. Our data suggest that the HopQ-CEACAM interaction contributes to gastric colonization or Hp-induced pathologies, although the precise role and functional consequences of this interaction in vivo remain to be determined.