The Early Adaptive Immune Response in the Pathophysiological Process of Pneumococcal Meningitis

Ribes, Sandra; Nessler, Stefan; Heide, Ev-Christin; Malzahn, Dörthe; Perske, Christina; Brüeck, Wolfgang und Nau, Roland (2017): The Early Adaptive Immune Response in the Pathophysiological Process of Pneumococcal Meningitis. In: Journal of infectious Diseases, Bd. 215, Nr. 1: S. 150-158

Abstract

Background. The adaptive immune system has been considered to play a minimal role in the early host response during bacterial meningitis. Methods. We investigated the progression and outcome of pneumococcal meningitis in Rag1(-/-) mice lacking functional B and T cells by assessing overall and symptom-free survival, bacteriological and histological studies, as well as flow cytometry and measurements of proinflammatory mediators. Results. The intracerebral injection of S. pneumoniae D39 induced the recruitment of B and T cells (CD4+, gamma delta and natural killer) into the brain of wild-type mice. Mice with no functional B and T cells developed clinical symptoms and succumbed to the infection earlier than the wild-type group. In the CNS, Rag1(-/-) mice showed lower levels of interleukin 1 beta, reduced microglial proliferation, and impaired granulocyte recruitment with an earlier spread of pneumococci into the bloodstream, compared with wild-type mice. Lack of B and T cells resulted in a severe impairment of bacterial clearance in blood, spleen, and liver and an exaggerated systemic inflammatory response. Conclusions. B and T cells are important effector cells delaying the spread of pneumococci from the brain to the systemic circulation and shaping the immune response, thereby prolonging the survival of the host in the absence of antibiotic treatment.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0022-1899
• Sprache: Englisch