Effects of antioxidants on DNA double-strand breaks in human gingival fibroblasts exposed to dental resin co-monomer epoxy metabolites

Yang, Yang; He, Xiuli; Shi, Jianwei; Hickel, Reinhard; Reichl, Franz-Xaver und Högg, Christof (2017): Effects of antioxidants on DNA double-strand breaks in human gingival fibroblasts exposed to dental resin co-monomer epoxy metabolites. In: Dental Materials, Bd. 33, Nr. 4: S. 418-426

Abstract

Objective. Eluted dental resin co-monomers can be metabolized to intermediate methacrylic acid (MA) and, further, to epoxy metabolites. Antioxidants have been studied previously, with the intention of decreasing the DNA double-strand breaks (DNA-DSBs) in human gingival fibroblasts (HGFs). In this study, the effects of the antioxidants, ascorbic acid (Asc) and N-acetylcysteine (NAC), were investigated on co-monomer metabolite-induced DNA-DSBs. Methods. HGFs were incubated with MA, 2,3-epoxy-2-methyl-propionicacid-methylester (EMPME) and 2,3-epoxy-2-methylpropionic acid (EMPA), respectively, in the presence or absence of antioxidants (Asc or NAC). EC so Values were obtained from an XTT-based viability assay. DNA-DSBs were determined using a y-H2AX assay. Results. The cytotoxicity of the compounds could be ranked in the following order (mean +/- SEM;n = 4): EMPA> EMPME > MA. The average number of DSBs-foci/cell induced by each substance at EC50-concentration could be ranked in the following order (mean +/- SD;n = 4): EMPA> EMPME> MA. EMPA (1.72 mM) and EMPME (2.58 mM) induced the highest number of DSBs-foci, that is 21-fold and 13-fold, respectively, compared to control (0.48 +/- 0.08 foci/cell). The addition of Asc (50;100;200 mu M) or NAC (50;100;200;500 mu M) to MA (15.64;5.21 mM), EMPME (2.58 mM), and EMPA (1.72;0.57 mM) significantly reduced the number of foci/cell in HGFs. The highest reduction could be found in HGFs with 1.72 mM EMPA, the addition of NAC (50;100;200;500 mu M) induced a 15-fold, 17-fold, 14-fold and 14-fold lower number of DSBs-foci/cell, respectively. Significance. Dental co-monomer epoxy metabolites, EMPME and EMPA, can induce DNADSBs. The addition of antioxidants (Asc or NAC) leads to reduction of DNA-DSBs, and NAC leads to more prominent reduction of DNA-DSBs compared to Asc. (C) 2017 The Academy of Dental Materials.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0109-5641
• Sprache: Englisch