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Gross, Stefanie; Erdmann, Michael; Händle, Ina; Voland, Steve; Berger, Thomas; Schultz, Erwin; Strasser, Erwin; Dankerl, Peter; Janka, Rolf; Schliep, Stefan; Heinzerling, Lucie; Sotlar, Karl; Coulie, Pierre; Schuler, Gerold and Schuler-Thurner, Beatrice (2017): Twelve-year survival and immune correlates in dendritic cell-vaccinated melanoma patients. In: Jci insight, Vol. 2, No. 8, e91438

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BACKGROUND. Reports on long-term (>= 10 years) effects of cancer vaccines are missing. Therefore, in 2002, we initiated a phase I/II trial in cutaneous melanoma patients to further explore the immunogenicity of our DC vaccine and to establish its long-term toxicity and clinical benefit after a planned 10-year followup. METHODS. Monocyte-derived DCs matured by TNF alpha, IL-1 beta, IL-6, and PGE 2 and then loaded with 4 HLA class I and 6 class II-restricted tumor peptides were injected intradermally in high doses over 2 years. We performed serial immunomonitoring in all 53 evaluable patients. RESULTS. Vaccine-specific immune responses including high-affinity, IFN gamma-producing CD4(+) and lytic polyfunctional CD8(+) T cells were de novo induced or boosted in most patients. Exposure of mature DCs to trimeric soluble CD40 ligand, unexpectedly, did not further enhance such immune responses, while keyhole limpet hemocyanin (KLH) pulsing to provide unspecific CD4(+) help promoted CD8(+) T cell responses - notably, their longevity. An unexpected 19% of nonresectable metastatic melanoma patients are still alive after 11 years, a survival rate similar to that observed in ipilimumab-treated patients and achieved without any major (>grade 2) toxicity. Survival correlated significantly with the development of intense vaccine injection site reactions, and with blood eosinophilia after the first series of vaccinations, suggesting that prolonged survival was a consequence of DC vaccination. CONCLUSIONS. Long-term survival in advanced melanoma patients undergoing DC vaccination is similar to ipilimumab-treated patients and occurs upon induction of tumor-specific T cells, blood eosinophilia, and strong vaccine injection site reactions occurring after the initial vaccinations.

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