Abstract
Interleukin 17 (IL-17) cytokines play a crucial role in host defense and inflammatory diseases. Of the six mammalian IL-17 members five are described in the chicken (gg) genome. A novel method that attached cytokines to the surface of cells via a GPI linker was established to generate two chicken IL-17A and one chicken IL-17F specific mab. Recombinant gg IL-17A and gg IL-17F that showed dimerization in Western blot were used to verify the antibodies specificity. The mab could detect gg IL-17 by intracellular cytokine staining as demonstrated on cells expressing recombinant IL-17. Furthermore IL-17A and lower amounts of IL-17F were detectable in CD4 positive T cells of stimulated splenocytes. In conclusion, we have generated novel tools to analyze chicken IL-17 in more detail and demonstrated that the surface expression of cytokines is a reliable method to generate specific mab applicable for intracellular cytokine staining.
Item Type: | Journal article |
---|---|
Faculties: | Veterinary Medicine |
Subjects: | 600 Technology > 610 Medicine and health |
ISSN: | 0145-305X |
Language: | English |
Item ID: | 52992 |
Date Deposited: | 14. Jun 2018, 09:51 |
Last Modified: | 04. Nov 2020, 13:31 |