Abstract
Chemical and biological tools are harnessed to investigate the impact of spatial factors for functional pairing of human lectins with counterreceptors. The homodimeric adhesion/growth-regulatory galectin-1 and a set of covalently linked homo-oligomers from di- to tetramers serve as proof-of-principle test cases. Glycodendrimersomes provide a versatile and sensitive diagnostic platform to reveal thresholds for ligand density and protein concentration in aggregation assays (trans-activity), irrespective of linker length between lectin domains. Monitoring the affinity of cell binding and ensuing tumor growth inhibition reveal the linker length to be a bidirectional switch for cis-activity. The discovery that two aspects of lectin functionality (trans-versus cis-activity) respond non-uniformly to a structural change underscores the power of combining synthetic and biological tools to advance understanding of the sugar functionality of the cell surface.
Item Type: | Journal article |
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Faculties: | Veterinary Medicine |
Subjects: | 600 Technology > 610 Medicine and health |
ISSN: | 1433-7851 |
Language: | English |
Item ID: | 53079 |
Date Deposited: | 14. Jun 2018, 09:52 |
Last Modified: | 04. Nov 2020, 13:31 |