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Göhring, Lutz S.; Brandes, K.; Ashton, L. V.; Wittenburg, L. A.; Olea-Popelka, F. J.; Lunn, D. P. and Hussey, G. Soboll (2017): Anti-inflammatory drugs decrease infection of brain endothelial cells with EHV-1 in vitro. In: Equine Veterinary Journal, Vol. 49, No. 5: pp. 629-636

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BackgroundEquine herpesvirus-associated myeloencephalopathy is the result of endothelial cell infection of the spinal cord vasculature with equine herpesvirus-1 (EHV-1) during cell-associated viraemia. Endothelial cell infection requires contact between infected peripheral blood mononuclear and endothelial cells. Inflammation generated during viraemia likely upregulates adhesion molecule expression on both cell types increasing contact and facilitating endothelial cell infection. ObjectivesEvaluating the role of anti-inflammatory drugs in decreasing endothelial cell infection with EHV-1. Study designIn vitro assay, crossover design, multiple drug testing. MethodsIn vitro modified infectious centre assay using immortalised carotid artery endothelial cells or primary brain endothelial cells with plaque counts per well as outcome. Cells were either anti-inflammatory drug treated or left untreated. ResultsSignificant reduction of plaque count when cells were treated compared with untreated cells. No dose-dependent effect when drug concentrations were increased to 10x dose. Treatment of both peripheral blood mononuclear cells (PBMC) and endothelial cells (EC) is required for significant plaque count reduction. Main limitationsIn vitro study. ConclusionsAnti-inflammatory drugs decrease infection of endothelial cells likely by reducing contact between EHV-1 infected PBMC and endothelial cells in vitro. The role of adhesion molecules in this process needs further investigation. In vitro results suggest anti-inflammatory drug therapy during EHV-1 infection and viraemia in horses could be clinically relevant.

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