Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ

Raddaoui, Nada; Stazzoni, Samuele; Möckl, Leonhard; Viverge, Bastien; Geiger, Florian; Engelke, Hanna; Bräuchle, Christoph und Carell, Thomas (2017): Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ. In: ChemBioChem, Bd. 18, Nr. 17: S. 1716-1720

Abstract

The in vivo incorporation of alkyne-modified bases into the genome of cells is today the basis for the efficient detection of cell proliferation. Cells are grown in the presence of ethinyl-dU (EdU), fixed and permeabilised. The incorporated alkynes are then efficiently detected by using azide-containing fluorophores and the Cu-I-catalysed alkyne-azide click reaction. For a world in which constant improvement in the sensitivity of a given method is driving diagnostic advancement, we developed azide- and alkyne-modified dendrimers that allow the establishment of sandwich-type detection assays that show significantly improved signal intensities and signal-to-noise ratios far beyond that which is currently possible.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department Chemie
• Fakultätsübergreifende Einrichtungen: Center for Integrated Protein Science Munich (CIPSM)
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• ISSN: 1439-4227
• Sprache: Englisch