Reduction of GAPDH in Lenses of Parkinson's Disease Patients: A Possible New Biomarker

Klettner, Alexa; Tholey, Andreas; Wiegandt, Alena; Richert, Elisabeth; Nölle, Bernhard; Deuschl, Guenther; Roider, Johann and Schneider, Susanne A. (2017): Reduction of GAPDH in Lenses of Parkinson's Disease Patients: A Possible New Biomarker. In: Movement Disorders, Vol. 32, No. 3: pp. 459-462

Abstract

Background: We previously showed that the protein pattern of lenses removed in cataract surgery differs between patients with Parkinson's disease and age-matched controls. In this study, we identified the protein reduced in abundance in the 34-to 37-kDa gel band. Methods: During cataract surgery (phacoemulsification), we collected the rinsing fluid and lens particles from the eyes of PD patients and controls. Residual lens fragments in the supernatant of 3 PD patients and aged-matched controls were studied for protein profiles using liquid chromatography-mass spectrometry and Western blots. Results: We identified glyceraldehyde-3-phosphate dehydrogenase by mass spectrometry as the protein reduced in abundance and verified this finding in Western blots. Conclusions: Glyceraldehyde-3-phosphate dehydrogenase has been implicated in PD development. The reduction of glyceraldehyde-3-phosphate dehydrogenase in the lenses of PD patients may be a new biomarker for PD and might also indicate an important role for this protein in PD development. (C) 2016 International Parkinson and Movement Disorder Society

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 0885-3185
• Language: English