Sodium channel inactivation kinetics of rat sensory and motor nerve fibres and their modulation by glutathione

Mitrović, N.; Quasthoff, Stefan und Grafe, Peter (1993): Sodium channel inactivation kinetics of rat sensory and motor nerve fibres and their modulation by glutathione. In: Pflügers Archiv European Journal of Physiology, Bd. 425, Nr. 5-6: 453 -461

Abstract

Na+ channel currents of rat motor and sensory nerve fibres were studied with the patch-clamp technique on enzymatically demyelinated axons. Differences between motor and sensory fibres in multi-channel inactivation kinetics and the gating of late single-channel currents were investigated. In the axon-attached mode, inactivation of multi-channel Na+ currents in sensory axons was best fitted with a single time constant while for motor axons two time constants were needed. Late single-channel currents in sensory axons were characterized by short openings whereas motor axons exhibited additional long single-channel openings. In contrast, in excised, inside-out membrane patches, no differences between motor and sensory fibres were found: in both types of fibre inactivation of multi-channel Na+ currents proceeded with two time constants and late single-channel currents showed short and long openings. After application of the reducing agent glutathione to the cytoplasmic side of excised inside-out patches, inactivation of Na+ currents in both motor and sensory fibres proceeded with a single, fast exponential time constant and late currents appeared with short openings only. These data indicate that the axonal metabolism may contribute to the different inactivation kinetics of Na+ currents in motor and sensory nerve fibres.

• Dokumententyp: Zeitschriftenartikel
• Keywords: Axon - Peripheral nerve - Patch clamp - Ion channels - Metabolism - Glutathione - Iodate - Monochlorobimane
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit