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Wegner, S.; Bauer, J. I.; Dietrich, R.; Märtlbauer, E.; Usleber, E.; Gottschalk, C.; Gross, M. (2017): A highly specific competitive direct enzyme immunoassay for sterigmatocystin as a tool for rapid immunochemotaxonomic differentiation of mycotoxigenic Aspergillus species. In: Letters in Applied Microbiology, Vol. 64, No. 2: pp. 124-130
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A simplified method to produce specific polyclonal rabbit antibodies against sterigmatocystin (STC) was established, using a STC-glycolic acid-ether derivative (STC-GE) conjugated to keyhole limpet haemocyanin (immunogen). The competitive direct enzyme immunoassay (EIA) established for STC had a detection limit (20% binding inhibition) of 130 pg ml(-1). The test was highly specific for STC, with minor cross-reactivity with O-methylsterigmatocystin (OMSTC, 0.87%) and negligible reactivity with aflatoxins (< 0.02%). STC-EIA was used in combination with a previously developed specific EIA for aflatoxins (< 0.1% cross-reactivity with STC and OMSTC), to study the STC/aflatoxin production profiles of reference strains of Aspergillus species. This immunochemotaxonomic procedure was found to be a convenient tool to identify STC- or aflatoxin-producing strains.