Expression pattern of STAT5A gene during early bovine embryogenesis

Flisikowski, Krzysztof; Boulhaeve, Marc; Lopes, Fabiola P.; Wolf, Eckhard und Zwierzchowski, Lech (2015): Expression pattern of STAT5A gene during early bovine embryogenesis. In: Zygote, Vol. 23, No. 2: pp. 307-311

Abstract

Growth hormone (GH) plays an important role in early embryo development. It has been shown to activate multiple pathways, the most comprehensively studied being the STAT/JAK (Signal transducers and activators of transcription/Janus kinase) pathway. The objective of the present study was to investigate STAT5A gene expression during early bovine embryogenesis. Real-time polymerase chain reaction (RT-PCR) was used to measure the abundance of STAT5A transcripts. The mRNA was present at all stages of preimplantation bovine embryos investigated. The most abundant STAT5A expression occurred at the 2-cell stage. Expression was markedly reduced between the 4-cell and 8-cell stages, coinciding with the known time of embryo genome activation and loss of maternal mRNAs. This finding suggests that the embryonic STAT5A gene is primarily activated by maternal gene products.

• Item Type: Journal article
• Faculties: Veterinary Medicine
• Subjects: 600 Technology > 610 Medicine and health
• Alliance/National Licence: This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
• Language: English