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Scully, Deidre; Dockery, Peter; Reese, Sven ORCID: 0000-0002-4605-9791; Kölle, Sabine (15. January 2019): How do ovarian cysts affect the microarchitexture of the oviduct? Winter Symposium of the Microscopy Society of Ireland, 15.-16. Januar 2019, University College Dublin, Dublin, Irland.
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Abstract

Ovarian cysts are a frequent cause of subfertility in humans and animals. However, the precise effects of follicular cysts, luteinised follicular cysts and luteal cysts on the microarchitecture of the tubes are still unknown. Using the bovine as a model, the aim of this study was to analyse the effects of ovarian cysts on the functional morphology of the oviduct. For this purpose, fallopian tubes obtained from cows with and without ovarian cysts were investigated using stereomicroscopy, scanning and transmission electron microscopy (SEM, TEM) and live cell imaging. Our studies revealed that cysts occurred on the right ovary in 67% of cows. Stereomicroscopy revealed enlarged primary folds in cows with ovarian cysts as compared to control cows in diestrus. In the tubal epithelium of cows with ovarian cysts the percentage of secretory cells as well as the synthesis of acidic mucopolysaccharides and glycoproteins was significantly increased as compared to controls {A NOVA, p<O.OS and p<O.OOl, respectively). As seen by TEM, the oviductal secretory cells of cows with ovarian cysts contained dilated rough endoplasmic reticulum as well as large amounts of electron dense secretory granules and heterolysosomes. Live cell imaging proved that the interaction between the oviduct and oocyte-cumuluscomplex, as well as the formation of the sperm reservoir, was similar to that seen in healthy control cows. Particle transport speed, which is an indicator for oocyte and embryo transport speed, was significantly lowered in the ipsilateral ampulla of cows with luteal cysts (MannWhitney, p<O.OS). Our results imply that the occurrence of ovarian cysts is correlated with reduced transport efficiency which may result in compromised gamete and embryo survival. Thus, innovative therapeutic strategies for treatment of ovarian cysts do not only require successful induction of ovulation and fertilisation, but also optimisation of nutrition and transport of the early embryo in the oviduct.