Dendrimer-based signal amplification of click-labelled DNA in situ

Raddaoui, Nada; Stazzoni, Samuele; Möckl, Leonhard; Viverge, Bastien; Geiger, Florian; Engelke, Hanna; Bräuchle, Christoph and Carell, Thomas (22. June 2017): Dendrimer-based signal amplification of click-labelled DNA in situ. In: ChemBioChem, Vol. 18, No. 17: pp. 1716-1720

Abstract

The in vivo incorporation of alkyne modified bases into the genome of cells is today the basis for efficient detection of cell proliferation. Cells are grown in the presence of ethinyl-dU (EdU), fixed and permeabilized. The incorporated alkynes are then efficiently detected using azide-containing fluorophores and the Cu(I) catalyzed alkyne-azide click reaction. In a world where constant improvement of the sensitivity of a given method is driving diagnostic advancement, we developed azide and alkyne modified dendrimers that allow to establish sandwich-type detection assays that show significantly improved signal intensities and signal to noise ratios far beyond of what is currently possible.

• Item Type: Journal article
• EU Funded Grant Agreement Number: 642023
• EU Projects: Horizon 2020 > Marie Skłodowska Curie Actions > Marie Skłodowska-Curie Innovative Training Networks > 642023: ClickGene - Click Chemistry for Future Gene Therapies to Benefit Citizens, Researchers and Industry
• Form of publication: Submitted Version
• Keywords: click chemistry; cell proliferation; dendrimer; fluorescence microscopy; high throughput screening
• Faculties: Chemistry and Pharmacy; Chemistry and Pharmacy > Department of Chemistry
• Research Centers: Center for Integrated Protein Science Munich (CIPSM)
• Subjects: 500 Science > 540 Chemistry; 500 Science > 570 Life sciences; biology
• ISSN: 1439-4227
• Language: English