Logo Logo
Switch Language to German

Michaelides, Iacovos N.; Tago, Nobuhiro; Viverge, Bastien and Carell, Thomas ORCID logoORCID: https://orcid.org/0000-0001-7898-2831 (14. September 2017): Synthesis of RNA containing 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine. In: Chemistry-A European Journal, Vol. 23, No. 63: pp. 15894-15898 [PDF, 654kB]


5-Hydroxymethyl-, 5-formyl-, and 5-carboxycytidine are new epigenetic bases (hmdC, fdC, cadC) that were recently discovered in the DNA of higher eukaryotes. The same bases (hmC, fC and caC) have now also been detected in mammalian RNA with a high abundance in mRNA. While for the DNA bases phosphoramidites (PAs) are available that allow the synthesis of xdC-containing oligonucleotides for deeper biological studies, the corresponding silyl protected RNA PAs for fC and caC have not yet been disclosed. Here we report novel RNA PAs for hmC, fC and caC that can be used in routine RNA synthesis. The new building blocks are compatible with the canonical PAs and also with themselves, which enables even the synthesis of RNA strands containing all three of these bases. The study will pave the way for detailed physical, biochemical and biological studies to unravel the function of these non-canonical modifications in RNA.

Actions (login required)

View Item View Item