Orchestrating the biosynthesis of an unnatural pyrrolysine amino acid for its direct incorporation into proteins inside living cells

Ehrlich, Michael; Gattner, Michael J.; Viverge, Bastien; Bretzler, Johanna; Eisen, David; Stadlmeier, Michael ; Vrabel, Milan; Carell, Thomas (2. April 2015): Orchestrating the biosynthesis of an unnatural pyrrolysine amino acid for its direct incorporation into proteins inside living cells. In: Chemistry-A European Journal, Vol. 21, No. 21: pp. 7701-7704

Abstract

We here report the construction of an E. coli expression system able to manufacture an unnatural amino acid by an artificial biosynthesis. This can be orchestrated with incorporation into protein by amber stop codon suppression inside a living cell. In our case an alkyne bearing pyrrolysine amino acid was biosynthesized and incorporated site-specifically allowing orthogonal double protein labeling.

• Item Type: Journal article
• Form of publication: Postprint
• Keywords: pyrrolysine; unnatural amino acid; amber suppression; bioorganic chemistry; synthetic biology
• Faculties: Chemistry and Pharmacy > Department of Chemistry
• Research Centers: Center for Integrated Protein Science Munich (CIPSM)
• Subjects: 500 Science > 540 Chemistry; 500 Science > 570 Life sciences; biology
• ISSN: 0947-6539
• Language: English