A sulfoxide-based isobaric labelling reagent for accurate quantitative mass spectrometry

Stadlmeier, Michael ; Bogena, Jana ; Wallner, Miriam; Wühr, Martin ; Carell, Thomas (8. January 2018): A sulfoxide-based isobaric labelling reagent for accurate quantitative mass spectrometry. In: Angewandte Chemie-international Edition, Vol. 57, No. 11: pp. 2958-2962

Abstract

Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist of a balancer and a reporter part that separate in the gas phase. An ingenious distribution of stable isotopes provides multiple reagents with identical molecular weight but a different mass of the reporter groups, allowing relative quantification of multiple samples in one measurement. Current generation reagents require a high fragmentation energy for cleavage, leading to incomplete fragmentation and hence loss of signal intensity. Here we report a new isobaric labelling reagent, where the balancer and the reporter are linked by a sulfoxide group, which, based on the sulfoxide pyrolysis, leads to easy and asymmetric cleavage at low fragmentation energy. The fragmentation of our new design is significantly improved, yielding more intense complementary ion signals, allowing complementary ion cluster analysis as well.

• Item Type: Journal article
• EU Funded Grant Agreement Number: 642023
• EU Projects: Horizon 2020 > Marie Skłodowska Curie Actions > Marie Skłodowska-Curie Innovative Training Networks > 642023: ClickGene - Click Chemistry for Future Gene Therapies to Benefit Citizens, Researchers and Industry
• Form of publication: Postprint
• Keywords: Peptide derivatization reagent; sulfoxide pyrolysis; isobaric labelling; quantitative mass spectrometry; complementary ion clusters; proteomics
• Faculties: Chemistry and Pharmacy > Department of Chemistry
• Research Centers: Center for Integrated Protein Science Munich (CIPSM)
• Subjects: 500 Science > 540 Chemistry; 500 Science > 570 Life sciences; biology
• ISSN: 1433-7851
• Language: English