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Abstract
Background: Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate the wound-healing process. Methods: We could collect wound fluid samples from 4 fingertip injuries and 7 split skin donor sites at the 5th day during dressing change. Blood serum samples served as controls. The proliferation rate of MSCs and fibroblasts (HS27) was continuously measured through impedance analysis for 60h and by Alamarblue analysis after 72h. Cell migration was evaluated continuously for 15h and confirmed by the in vitro wound-healing assay. Results: Migration of MSCs under the influence of both wound fluids was significantly faster than controls from 4 to 6h after incubation and reversed after 9h. MSC proliferation in wound fluid groups showed a significant increase at 5 and 10h and was significantly decreased after 45h. Fibroblasts in wound fluid groups showed overall a significant increase in migration and a significant decrease in proliferation compared to controls. Conclusion: OD-induced secretomes influence MSCs and fibroblasts and thereby possibly modulate wound healing and scar tissue formation.
| Dokumententyp: | Zeitschriftenartikel |
|---|---|
| Fakultät: | Medizin |
| Themengebiete: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit |
| URN: | urn:nbn:de:bvb:19-epub-63254-5 |
| ISSN: | 0949-2321 |
| Sprache: | Englisch |
| Dokumenten ID: | 63254 |
| Datum der Veröffentlichung auf Open Access LMU: | 19. Jul. 2019, 12:12 |
| Letzte Änderungen: | 04. Nov. 2020, 13:41 |
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