Abstract
The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (C-Tmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The C-Tmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that C-Tmin readouts of decontaminated sputum samples should be interpreted with caution.
| Item Type: | Journal article |
|---|---|
| Faculties: | Medicine |
| Subjects: | 600 Technology > 610 Medicine and health |
| ISSN: | 1472-9792 |
| Language: | English |
| Item ID: | 63448 |
| Date Deposited: | 19. Jul 2019, 12:13 |
| Last Modified: | 04. Nov 2020, 13:41 |
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