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Ishida, Yuko; Kuninaka, Yumi; Furukawa, Fukumi; Kimura, Akihiko; Nosaka, Mizuho; Fukami, Mie; Yamamoto, Hiroki; Kato, Takashi; Shimada, Emi; Hata, Satoshi; Takayasu, Tatsunori; Eisenmenger, Wolfgang and Kondo, Toshikazu (2018): Immunohistochemical analysis on aquaporin-1 and aquaporin-3 in skin wounds from the aspects of wound age determination. In: International Journal of Legal Medicine, Vol. 132, No. 1: pp. 237-242

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Immunohistochemical investigation of aquaporin (AQP)1 and AQP3 was performed in human skin wounds obtained from forensic autopsy cases. A total of 55 human skin wounds of different postinfliction intervals were collected as follows: group I, 0-3 days (n = 16);II, 4-7 days (n = 11);III, 9-14 days (n = 16);and IV, 17-21 days (n = 12). In uninjured skin samples, AQP1 and AQP3 could be slightly detected in dermal vessels and keratinocytes, respectively. The percentage of AQP1(+) vessels and the number of AQP3(+) keratinocytes were apparently elevated in accordance with wound ages. The number of AQP3(+) keratinocytes was distinctly evident in groups II and III. Morphometrically, both AQP1(+) vessel area and AQP3(+) cell number were markedly increased in group II, compared with other three groups. With regard to forensic safety, AQP1(+) vessel area of over 5% would imply wound ages of 4-12 days. Moreover, the positive area of > 15% would suggest wound age of 7-10 days. Especially, most samples of skin wounds aged 5-10 days except for only one sample (a 10-day-old wound) showed AQP3(+) cell number of > 300, and the remaining other samples had that of < 300. Thus, the AQP3(+) cell number of > 300 would indicate wound ages of 5-10 days. Collectively, immunohistochemical analyses of AQP1 and AQP3 in human skin wounds would support the objective accuracy of wound age determination.

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