Platelet Migration and Bacterial Trapping Assay under Flow

Fan, Shuxia; Lorenz, Michael; Massberg, Steffen and Gaertner, Florian (2018): Platelet Migration and Bacterial Trapping Assay under Flow. In: Bio-Protocol, Vol. 8, No. 18, e3018

Abstract

Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets use their ability to migrate to collect and bundle fibrin (ogen)-bound bacteria accomplishing efficient intravascular bacterial trapping. Here, we describe a method that allows analyzing platelet migration in vitro, focusing on their ability to collect bacteria and trap bacteria under flow.

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• Language: English