Progesterone and cyclic adenosine monophosphate down‐regulate CD90 in the stromal cells of human decidua. In vitro evidence and in situ findings

Vinketova, Kameliya; Karagyozov, Iliya; Koleva, Vesselina; Hristov, Michael; Mourdjeva, Milena; Sperandio, Markus und Oreshkova, Tsvetelina (2018): Progesterone and cyclic adenosine monophosphate down‐regulate CD90 in the stromal cells of human decidua. In vitro evidence and in situ findings. In: American Journal of Reproductive Immunology, Bd. 80, Nr. 5, e13043

Abstract

Objective: CD90 is a glycoprotein involved in leukocyte relocation and cell differentiation. CD90 is expressed in endothelial and stromal cells in human endometrium;however, its role in the remodeling of the decidual tissue during pregnancy is poorly understood. Here, we investigate how CD90 expression in decidual stromal cells (DSCs) is regulated. Method of Study: The native CD90 receptor in stromal cells in decidua was investigated via histology. We further develop in vitro culture of DSCs which allows us to test the effects of hormones and paracrine signals on CD90 expression. Results: Stromal cells in first-trimester human decidua display heterogeneous levels of CD90 expression. In vitro analyses reveal that progesterone, a factor normally secreted by trophoblast cells in the placenta, and extracellular cyclic adenosine monophosphate, a known downstream signaling messenger of progesterone, reduce CD90 expression in DSCs by similar to 30%. This reduction in CD90 expression correlates with a change toward a more highly differentiated cell state. Conclusion: DSCs in early pregnancy show different levels of CD90 expression, suggesting different DSC differentiation and selective interactions with cells during decidual morphogenesis.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1046-7408
• Sprache: Englisch