Chicken IL-17A is expressed in alpha beta and gamma delta T cell subsets and binds to a receptor present on macrophages, and T cells

Walliser, Isabelle und Göbel, Thomas W. (2018): Chicken IL-17A is expressed in alpha beta and gamma delta T cell subsets and binds to a receptor present on macrophages, and T cells. In: Developmental and Comparative Immunology, Bd. 81: S. 44-53

Abstract

IL-17A as important cytokine in host defense has been analysed intensively and various homologous have been identified. To further gain insight into the functional properties of chicken (gg) IL-17A its expression profile was analysed by intracellular cytokine staining. In splenocytes and peripheral blood mononuclear cells gg IL-17A was detected in subsets of CD4(+) T cells and gamma delta T cells. In contrast the gg IL-17A producing populations in intestinal intraepithelial lymphocytes were characterized as either CD3(+)CD25(+) cells or gamma delta T cells. Furthermore, using FLAG tagged gg IL-17A, binding to its receptor was demonstrated on the macrophage cell line HD11. In peripheral blood IL-17A binding activity was found on zeta beta and gamma delta T cell subsets, monocytes and a distinct population of CD25(high) cells. Treatment of HD11 cells with gg IL-17A induced IL-6 mRNA expression and nitric oxide production. These results demonstrate the presence of alpha beta T helper(17) cell subset and IL-17 producing gamma delta T cells in the chicken.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Tiermedizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0145-305X
• Sprache: Englisch