Abstract
Mammalian type I interferons (IFN alpha/beta) are known to modulate inflammatory processes in addition to their antiviral properties. Indeed, virus-induced type I interferons regulate the mammalian phagocyte immune response to bacteria during superinfections. However, it remains unresolved whether type I IFNs similarly impact the chicken macrophage immune response. We first evidenced that IFN alpha and IFN beta act differently in terms of gene expression stimulation and activation of intracellular signaling pathways in chicken macrophages. Next, we showed that priming of chicken macrophages with IFN alpha increased bacteria uptake, boosted bacterial-induced ROS/NO production and led to an increased transcriptional expression or production of NOS2/NO, IL1B/IL-1 beta, and notably IFNB/IFN beta Neutralization of IFN beta during bacterial challenge limited IFN alpha-induced augmentation of the pro-inflammatory response. In conclusion, we demonstrated that type I IFNs differently regulate chicken macrophage functions and drive a pro inflammatory response to bacterial challenge. These findings shed light on the diverse functions of type I IFNs in chicken macrophages.
Item Type: | Journal article |
---|---|
Faculties: | Veterinary Medicine |
Subjects: | 600 Technology > 610 Medicine and health |
ISSN: | 0145-305X |
Language: | English |
Item ID: | 65800 |
Date Deposited: | 19. Jul 2019, 12:18 |
Last Modified: | 04. Nov 2020, 13:46 |